Anthocyanin attenuates oxygen-glucose deprivation/reperfusion-induced apoptosis of PC12 cells via inactivation of ASK1/JNK/p38 signaling pathway

Purpose: To investigate whether the cytoprotective effect of anthocyanin (Anc) on oxygen-glucose deprivation/reperfusion (OGD/R)-induced cell injury is related to apoptosis signal-regulating kinase 1 (ASK1)/c-Jun N-terminal (JNK)/p38 signaling pathway.
 Methods: PC12 cells were pre-treated with various concentrations Anc (10, 50, and 100 ?g/mL) in OGD/R-induced model. The 3-(4, 5)-dimethylthiahiazo (-z-y1)-3, 5-di-phenytetrazoliumromide (MTT) assay was used assess viability. Cell measured by lactic acid dehydrogenase (LDH) release flow cytometry. Western blot employed determine protein expressions BCL-2, BAX, caspase-3, p-ASK1 (Thr845), p-JNK, p-p38.
 Results: results indicate that increased viability after OGD/R exposure (p < 0.05), also efficiently rescued 0.05). Mechanistic studies showed these protective roles are inhibition ASK1/JNK/p38 Conclusion: protects against enhancing inhibiting apoptosis. underlying mechanism action partly via inactivation pathway. Thus, has promise as a potential natural agent prevent treat cerebral ischemia-reperfusion injury.